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KMID : 0545120230330010028
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Journal of Microbiology and Biotechnology
2023 Volume.33 No. 1 p.28 ~ p.34
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Crystal Structure of the Metallo-Endoribonuclease YbeY from Staphylococcus aureus
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Lee Jin-Wook
Jo In-Seong
Kwon Ae-Ran
Ha Nam-Chul
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Abstract
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Endoribonuclease YbeY is specific to the single-stranded RNA of ribosomal RNAs and small RNAs. This enzyme is essential for the maturation and quality control of ribosomal RNA in a wide range of bacteria and for virulence in some pathogenic bacteria. In this study, we determined the crystal structure of YbeY from Staphylococcus aureus at a resolution of 1.9 A in the presence of zinc chloride. The structure showed a zinc ion at the active site and two molecules of tricarboxylic acid citrate, which were also derived from the crystallization conditions. Our structure showed the zinc ion-bound local environment at the molecular level for the first time. Molecular comparisons were performed between the carboxylic moieties of citrate and the phosphate moiety of the RNA backbone, and a model of YbeY in complex with a single strand of RNA was subsequently constructed. Our findings provide molecular insights into how the YbeY enzyme recognizes single-stranded RNA in bacteria.
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KEYWORD
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Staphylococcus aureus, YbeY, metalloprotein, endoribonuclease, zinc, crystal structure
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